Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus
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چکیده
منابع مشابه
Isolation and Characterization of Polygalacturonase Produced by Tetracoccosporium sp.
Thirty-five fungal strains which isolated from vegetable wastes, were screened for the use of polygalacturonic acid as the sole carbon source. Twenty-five isolates were positive for polygalacturonase activity in cup-plate assay, as evidenced by clear hydrolysation zones. The most productive strain was determined by measuring clear zones formed around colonies stained with ruthenium red.</em...
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Introduction: Production of a novel polygalacturonase (PG) active at pH = 3.0 and suitable to be used in fruit juice industries from Macrophomina phaseolina was evaluated. Suitable carbon, nitrogen and phosphorous forms were determined and the condition was optimized for higher PG production. Materials and methods: Macrophomina phaseolina was cultured in so called production medium. The secreto...
متن کاملPurification and biochemical characterization of polygalacturonase produced by penicillium expansum during postharvest decay of 'Anjou' pear.
A polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetect...
متن کاملisolation and characterization of polygalacturonase produced by tetracoccosporium sp.
thirty-five fungal strains which isolated from vegetable wastes, were screened for the use of polygalacturonic acid as the sole carbon source. twenty-five isolates were positive for polygalacturonase activity in cup-plate assay, as evidenced by clear hydrolysation zones. the most productive strain was determined by measuring clear zones formed around colonies stained with ruthenium red. the hig...
متن کاملIsolation, purification, and characterization of a polygalacturonase produced in Penicillium solitum-decayed 'Golden Delicious' apple fruit.
Polygalacturonase (PG) was extracted and purified from decayed 'Golden Delicious' apple fruit inoculated with Penicillium solitum. Ammonium sulfate, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. The purified PG most likely originates from the fungus because PG activity from hea...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2014
ISSN: 1516-8913
DOI: 10.1590/s1982-88372014000100017